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KMID : 0368419960390020093
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Journal of Plant Biology
1996 Volume.39 No. 2 p.93 ~ p.98
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Improved in vitro Regeneration of Potato(Solanum tuberosum cv. Superior) Transformed by Agrobacterium Expressing β-Glucuronidase
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Park, Yoonkyung
Park, Genesue/Yang, Young Ki/Cheong, Hyeon Sook
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Abstract
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In order to enhance the system of potato transformation and further regeneration, potato was transformed using the Agrobacterium tumefaciens harboring β-glucuronidase (GUS) gene. We found that a series of modified medium attained 100% shoot regeneration within 5 weeks after the preincubated explants on stage Ⅰ medium were infected with Agrobacterium. Callus appeared at the cut edges of stem segments on stage Ⅱ medium, mainly at the basal parts. Some explants started to form shoots after two to three weeks on stage Ⅲ medium containing kanamycin (50 ㎎/L). When transferred to MS medium containing 200 ㎎/L kanamycin, 81% of the transformed shoots formed roots at the cut edge of the plantlets. In contrast, untransformed shoots never rooted and became yellowish after few weeks under the same conditions. Southern and northern analysis indicated that transformation of potatoes was successfully proceeded. Consequently, we have improved in vitro shoot regeneration on the callus derived from the potato explants, which were incubated with Agrobacteria. The regeneration cycle was shortened after the transformation and finally the transformation efficiency was highly enhanced.
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